diff --git a/test/browser_common.py b/test/browser_common.py
index 8d15aa1e295b6..833e4a86981f5 100644
--- a/test/browser_common.py
+++ b/test/browser_common.py
@@ -205,6 +205,21 @@ def find_system_firefox_platform_ini():
   return version
 
 
+@memoize
+def get_chrome_version():
+  if not is_chrome():
+    return UNSUPPORTED
+  exe = shlex.split(EMTEST_BROWSER, posix=not WINDOWS)[0]
+  if WINDOWS:
+    cmd = ['powershell', '-NoProfile', '-Command', f'(Get-Item "{exe}").VersionInfo.ProductVersion']
+  else:
+    if exe.endswith('.app'):
+      exe = os.path.join(exe, 'Contents/MacOS/Google Chrome')
+    cmd = [exe, '--version']
+  version = subprocess.check_output(cmd, text=True)
+  return int(re.search(r'\b(\d+)\.', version).group(1))
+
+
 def browser_should_skip_feature(skip_env_var, feature):
   # If an env. var. EMTEST_LACKS_x to skip the given test is set (to either
   # value 0 or 1), don't bother checking if current browser supports the feature
@@ -243,6 +258,7 @@ class ChromeConfig:
     # this flag for now: https://crbug.com/638180
     '--no-first-run -start-maximized --no-sandbox --enable-unsafe-swiftshader --use-gl=swiftshader --enable-features=JavaScriptSourcePhaseImports',
     '--enable-experimental-webassembly-features',
+    '--enable-blink-features=AudioWorkletSharedPort',
     # The runners lack sound hardware so fallback to a dummy device (and
     # bypass the user gesture so audio tests work without interaction)
     '--use-fake-device-for-media-stream --autoplay-policy=no-user-gesture-required',
diff --git a/test/test_browser.py b/test/test_browser.py
index ce35e41905e63..15f8629956415 100644
--- a/test/test_browser.py
+++ b/test/test_browser.py
@@ -31,6 +31,7 @@
   browser_should_skip_feature,
   find_browser_test_file,
   get_browser,
+  get_chrome_version,
   get_firefox_version,
   get_safari_version,
   is_chrome,
@@ -181,6 +182,7 @@ def decorator(min_required_version, note=''):
 
 requires_safari_version = requires_version('safari', get_safari_version)
 requires_firefox_version = requires_version('firefox', get_firefox_version)
+requires_chrome_version = requires_version('chrome', get_chrome_version)
 
 
 def is_jspi(args):
@@ -5434,6 +5436,15 @@ def test_audio_worklet(self, args):
       self.skipTest('This test requires a browser with ES6 Module Workers support')
     self.btest_exit('webaudio/audioworklet.c', cflags=['-sAUDIO_WORKLET', '-sWASM_WORKERS', '-DTEST_AND_EXIT'] + args)
 
+  # Tests that AudioWorklet.port (spec-defined shared MessagePort) can be used
+  # without falling back to the em-bootstrap AudioWorkletProcessor.
+  @requires_sound_hardware
+  @no_safari('https://bugs.webkit.org/show_bug.cgi?id=299386')
+  @requires_firefox_version(138)
+  @requires_chrome_version(155, 'https://crbug.com/40210558')
+  def test_audio_worklet_port(self):
+    self.btest_exit('webaudio/test_audio_worklet_port.c', cflags=['-sAUDIO_WORKLET', '-sWASM_WORKERS'])
+
   # Tests that audioworklets and workers can be used at the same time
   # Note: doesn't need audio hardware (and has no AW code that tests 2GB or wasm64)
   def test_audio_worklet_worker(self):
diff --git a/test/webaudio/test_audio_worklet_port.c b/test/webaudio/test_audio_worklet_port.c
new file mode 100644
index 0000000000000..d35dc2ee8147e
--- /dev/null
+++ b/test/webaudio/test_audio_worklet_port.c
@@ -0,0 +1,33 @@
+#include <emscripten/webaudio.h>
+#include <assert.h>
+#include <stdint.h>
+
+uint8_t audio_worklet_stack[4096];
+EMSCRIPTEN_WEBAUDIO_T audio_context;
+
+void do_exit() {
+  emscripten_destroy_audio_context(audio_context);
+  emscripten_force_exit(0);
+}
+
+void in_audio_worklet() {
+  assert(emscripten_current_thread_is_audio_worklet());
+  emscripten_audio_worklet_post_function_v(EMSCRIPTEN_AUDIO_MAIN_THREAD, do_exit);
+}
+
+void worklet_init(EMSCRIPTEN_WEBAUDIO_T ctx, bool success, void* userData) {
+  assert(success);
+  EM_ASM({
+    var audio = emscriptenGetAudioObject($0);
+    assert(audio.audioWorklet.port instanceof MessagePort);
+    assert(!audio.audioWorklet.bootstrapMessage);
+  }, ctx);
+  emscripten_audio_worklet_post_function_v(ctx, in_audio_worklet);
+}
+
+int main() {
+  audio_context = emscripten_create_audio_context(0);
+  emscripten_start_wasm_audio_worklet_thread_async(
+    audio_context, audio_worklet_stack, sizeof(audio_worklet_stack), worklet_init, 0);
+  emscripten_exit_with_live_runtime();
+}